T4 DNA Ligase from New England Biolabs

Product Specs
ItemT4 DNA Ligase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0202M
Quantity100000 units
Concentration30 u/µl
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

Specifications/Features

Unit Definition:
One NEB unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of Lambda DNA in 30 minutes at 16°C in 20 µl of the above assay mixture and a 5´ DNA terminiconcentration of 0.12 µM (300 µg/ml).

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) High-throughput functional analysis of regulatory variants using a massively parallel reporter assaySTAR ProtocolsDecember 1, 2023Kate Delfosse, Chiara Gerhardinger, John L. Rinn, Philipp G. Maassv3,Illumina,Cat#FC-110-3001 SfiI,NEB,Cat#R0123L "Alkaline phosphatase, calf intestinal",NEB,Cat#M0525 T4 DNA ligase,NEB,Cat#M0202M T4 DNA ligase reaction buffer,NEB,Cat#B0202S XbaI,NEB,Cat#R0145L KpnI-HF,NEB,Cat#R3142L dNTP solution

(2) Genome folding principles revealed in condensin-depleted mitotic chromosomesbioRxivNovember 17, 2023Han Zhao, Yinzhi Lin, En Lin, Fuhai Liu, Lirong Shu, Dannan Jing, Baiyue Wang, Manzhu Wang, Fengnian Shan, Lin Zhang, et al.DNA Polymerase I, Large (Klenow) fragment (NEB, Cat#M0210). Chromatin was then ligated in-situ with 2000U T4 DNA ligase (NEB, Cat#M0202M) for 4 hours at 16°C followed by further incubation for 2 hours at room temperature. Chromatin was then

(3) A dual sgRNA library design to probe genetic modifiers using genome-wide CRISPRi screensBMC GenomicsOctober 30, 2023Alina Guna, Katharine R. Page, Joseph M. Replogle, Theodore K. Esantsi, Maxine L. Wang, Jonathan S. Weissman, Rebecca M. Voorheesrecovery of both elements, either NT of EMC2 guide containing inserts were T4 ligated (ensure it is NEB #M0202M) with an insert to vector ratio of 1:2 for 16 h at 16 C. Various vector:insert molar ratios were tested

(4) Effective clearance of rituximab-resistant tumor cells by breaking the mirror-symmetry of immunoglobulin G and simultaneous binding to CD55 and CD20Scientific ReportsOctober 25, 2023Sang Min Lee, Sung-Won Min, Hyeong Sun Kwon, Gong-Deuk Bae, Ji Hae Jung, Hye In Park, Seung Hyeon Lee, Chung Su Lim, Byoung Joon Ko, Ji Chul Lee, et al.were synthesized by Cosmogentech. Vent polymerase, restriction enzymes, and T4 DNA ligase (Cat. No. M0202M) were purchased from New England Biolabs. The B-cell lymphoma (WSU-NHL) and Burkitt lymphoma (BJAB)

(5) High throughput PRIME editing screens identify functional DNA variants in the human genomebioRxivJuly 18, 2023Xingjie Ren, Han Yang, Jovia L. Nierenberg, Yifan Sun, Jiawen Chen, Cooper Beaman, Thu Pham, Mai Nobuhara, Maya Asami Takagi, Vivek Narayan, et al.resulting plasmid DNA was linearized by BsmbI digestion, gel-purified, and ligated using T4 ligase (NEB, M0202M) to a DNA fragment containing an sgRNA scaffold and the human U6 promoter. The resulting library was

(6) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.,STEMCELL Technologies,Cat#05010 SfiI,NEB,Cat#R0123L "Alkaline Phosphatase, Calf Intest",NEB,Cat#M0290L T4 DNA Ligase,NEB,Cat#M0202M XbaI,NEB,Cat#R0145L KpnI-HF,NEB,Cat#R3142L Q5- High Fidelity DNA polymerase,NEB,Cat#M0491L Formamide (Deionized

(7) AAV11 enables efficient retrograde targeting of projection neurons and enhances astrocyte-directed transductionNature CommunicationsJune 26, 2023Zengpeng Han, Nengsong Luo, Wenyu Ma, Xiaodong Liu, Yuxiang Cai, Jiaxin Kou, Jie Wang, Lei Li, Siqi Peng, Zihong Xu, et al.vector (Addgene, Watertown, MA, USA, 112862) using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA, M0202M). The recombinant plasmid was then transformed into chemically competent E. coli stain Stbl3. The positive

(8) How chromosomal translocations arise to cause cancer: Gene proximity, trans-splicing, and DNA end joiningiScienceJune 16, 2023Patrick Streb, Eric Kowarz, Tamara Benz, Jennifer Reis, Rolf Marschalek20% Triton X-100 was added and incubated for 1 h at 37°C while shaking gently. 100 U T4 DNA ligase (M0202M, NEB) was added and incubated for 4h at 16°C, followed by 30 min at room temperature. Finally, 30 μl

(9) A protocol to study three-dimensional genome structure in individual mutant preimplantation mouse embryosSTAR ProtocolsJune 1, 2023Daniel Gimenez-Llorente, Marta Portela, Ana Losada, Miguel Manzanares, Maria Jose Andreu(100 mM each) 11969064001,Sigma,Cat# 11969064001 T4 DNA Ligase (2000 U/mL),New England Biolabs,Cat# M0202M Dynabeads MyOne Streptavidin C1,Thermo Fisher,Cat# 65001 AluI,New England Biolabs,Cat# R0137S T4

(10) Regulatory architecture of housekeeping genes is driven by promoter assembliesCell reportsMay 30, 2023Dejosez M, Dall'Agnese A, Ramamoorthy M, Platt J, Yin X, Hogan M, Brosh R, Weintraub AS, Hnisz D, Abraham BJ, et al.buffer supplemented with 0.83% Triton X-100 and 0.1 mg/mL BSA. T4 DNA ligase (2.5 μL, 2000 U/μl; NEB, M0202M) was added and samples were incubated for 6 h at room temperature. Nuclei were collected and resuspended

(11) Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variantsCell ReportsMay 30, 2023Jianbo Wu, Zhenguo Chen, Yidan Gao, Zegen Wang, Jiarong Wang, Bing-Yu Chiang, Yunjiao Zhou, Yuru Han, Wuqiang Zhan, Minxiang Xie, et al.Scientific,Cat#M0530L T4 DNA polymerase,New England BioLabs,Cat#M0203L T4 DNA ligase,New England BioLabs,Cat#M0202M NucleoSpin Gel and PCR Clean-up,New England BioLabs,Cat#740690.250 Luciferase Cell Culture Lysis 5×

(12) Genomic Characterization of Carbapenem-Resistant Bacteria from Beef Cattle FeedlotsAntibiotics (Basel)May 25, 2023Sani-e-Zehra Zaidi, Rahat Zaheer, Krysty Thomas, Sujeema Abeysekara, Travis Haight, Luke Saville, Matthew Stuart-Edwards, Athanasios Zovoilis, Tim A. McAllisterrecovered DNA using Adapter Mix II Expansion kit EXP-AMII001 (ONT) along with the 5 µL T4 DNA ligase [Cat: M0202M; New England Biolabs (NEB) Ltd. Whitby, ON, Canada] and 10 µL quick ligation buffer [Cat: B6058S; New England Biolabs

(13) A tissue injury sensing and repair pathway distinct from host pathogen defenseCellMay 11, 2023Liu S, Hur YH, Cai X, Cong Q, Yang Y, Xu C, Bilate AM, Gonzales KAU, Parigi SM, Cowley CJ, et al.(Invitrogen, AM9937), and incubated with a ligation reaction mixture (10 unit/μL T4 DNA ligase (NEB, M0202M), 1x T4 DNA ligase buffer, 0.4 μg/μL BSA, 0.4 unit/μL RNaseOUT (Invitrogen, 10-777-019), 250 mM NaCl,

(14) Combinatorial quantification of 5mC and 5hmC at individual CpG dyads and the transcriptome in single cells reveals modulators of DNA methylation maintenance fidelitybioRxivMay 11, 2023Alex Chialastri, Saumya Sarkar, Elizabeth E. Schauer, Shyl Lamba, Siddharth S. Deyligation mix (1.11x T4 ligase reaction buffer, 4.44 mM ATP (NEB, P0756L), 2000 U T4 DNA ligase (NEB, M0202M)) was added to each sample, and the samples were incubated at 16°C for 16 hours. For H-M-Dyad-seq and

(15) Multiplexed long-read plasmid validation and analysis using OnRampGenome ResearchMay 8, 2023Camille Mumm, Melissa L. Drexel, Torrin L. McDonald, Adam G. Diehl, Jessica A. Switzenberg, Alan P. BoyleOne half volume of ligation buffer (4× T4 ligase buffer, 60% PEG 8000), 5 µL of T4 DNA ligase (NEB M0202M), and 2.5 µL of AMX (ONT SQK-LSK109) was added to the plasmid mixture and then incubated on a tube rotator

(16) PRC2.1- and PRC2.2-specific accessory proteins drive recruitment of different forms of canonical PRC1Molecular CellMay 4, 2023Eleanor Glancy, Cheng Wang, Ellen Tuck, Evan Healy, Simona Amato, Hannah K. Neikes, Andrea Mariani, Marlena Mucha, Michiel Vermeulen, Diego Pasini, et al.Kit,Qiagen,Cat#74104 RNase-Free DNase Set,Qiagen,Cat#79254 T4 DNA ligase,New England Biolabs,Cat#M0202M T4 DNA ligase buffer,New England Biolabs,Cat#B0202S High Sensitivity D1000 Reagents,Agilent,Cat#5067-5585 High

(17) Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustionMolecular cellApril 20, 2023Battistello E, Hixon KA, Comstock DE, Collings CK, Chen X, Rodriguez Hernaez J, Lee S, Cervantes KS, Hinkley MM, Ntatsoulis K, et al.ligated with 25ng of digested vector for 1h at room temperature with T4 ligase (New England Biolabs, Cat# M0202M). Following transformation in Stbl3 cells, single colonies were analyzed by Sanger sequencing using

(18) Application of a Spacer-nick Gene-targeting Approach to Repair Disease-causing Mutations with Increased SafetyBIO-PROTOCOLApril 20, 2023Ngoc Tung Tran, Mikhail Lebedin, Eric Danner, Ralf Kühn, Klaus Rajewsky, Van Trung Chunumber: 22700025)Carbenicillin (Sigma, catalog number: C1613-1ML)T4 DNA ligase (NEB, catalog number: M0202M)P3 Primary Cell electroporation buffer (see Recipes)Completed serum-free StemSpanTM SFEM II medium (see

(19) An RNA-based system to study hepatitis B virus replication and evaluate antiviralsScience advancesApril 14, 2023Yu Y, Schneider WM, Kass MA, Michailidis E, Acevedo A, Pamplona Mosimann AL, Bordignon J, Koenig A, Livingston CM, van Gijzel H, et al.catalog no. R3193L) and Not I–HF (NEB, catalog no.R3189L), ligated with T4 DNA ligase (NEB, catalog no. M0202M) into the gtA plasmid, and transformed into XL1-Blue electrocompetent bacteria (Agilent, catalog no.

(20) Overexpression of Krüppel-Like Factor 9 Enhances the Antitumor Properties of Paclitaxel in Malignant Melanoma-Derived Cell LinesPharmaceuticals (Basel)April 6, 2023Mohammed O. Altonsy, George X. Song-Zhao, Mahmoud M. Mostafa, Paule Régine Mydlarskiwas digested using the same enzymes, and both fragments were ligated using T4 DNA ligase (catalog no. M0202M; New England Biolabs) to generate pShuttle-CMV-KLF9. The ability of this vector to overexpress KLF9

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(1)

4/18/2016DNA Ligation Kit Benjamin GerovacThis is a good kit for DNA ligation. Good for ligating sticky and blunt ends with overnight incubation. Ligated DNA successfully transforms into bacteria.

Production of a Subtracted cDNA Library
July 01, 2001
Ligation of Linkers or Adapters to Double- Stranded cDNA
January 01, 1991

T4 DNA Ligase

T4 DNA Ligase from New England Biolabs

Specifications/Features

New England Biolabs

Citations (157)

Reviews

Protocols

Production of a Subtracted cDNA Library

Ligation of Linkers or Adapters to Double- Stranded cDNA

T4 DNA Ligase from New England Biolabs

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